MA5-27619
antibody from Invitrogen Antibodies
Targeting: HIF1AN
DKFZp762F1811, FIH1, FLJ20615, FLJ22027
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-27619 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HIF1AN Monoclonal Antibody (FIH 162c)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- FIH 162c
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HIF1AN in human cell lysates with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with HIF1AN monoclonal antibody (Product # MA5-27619) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of HIF1AN was achieved by transfecting HCT 116 with HIF1AN specific siRNAs (Silencer® select Product # s31198, s31197). Western blot analysis (Fig. a) was performed using whole cell extracts from the HIF1AN knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HIF1AN Monoclonal Antibody (FIH 162c) (Product # MA5-27619, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HIF1AN.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-HIF1AN Monoclonal Antibody (FIH 162c) (Product # MA5-27619) and a band at 40 kDa corresponding to HIF1AN was observed across the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), MCF7 (Lane 3), HCT 116 (Lane 4), LNCaP (Lane 5), DU 145 (Lane 6), HEK-293 (Lane 7) and K-562 (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).