PA5-117017

antibody from Invitrogen Antibodies

Targeting: HIF1AN DKFZp762F1811, FIH1, FLJ20615, FLJ22027

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117017

Provider

Invitrogen Antibodies

Product name

HIF1AN Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: MCF7, K-562, U-937, 293T, Mouse heart, Mouse skeletal muscle Immunogen sequence: MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF LYYDEKKMAN FQNFKPRSNR EEMKFHEFVE KLQDIQQRGG EERLYLQQTL NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY DEQQNFFAQI KGYKRCILFP PDQFECLYPY PVHHPCDRQS QVDFDNPDYE RFPNFQNVVG YETVVGPGDV LYIPMYWWHH IESLLNGGIT ITVNFWYKGA PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.57 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of HIF1AN in L929 cells using HIF1AN Polyclonal Antibody (Product # PA5-117017) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of HIF1AN in L929 cells using HIF1AN Polyclonal Antibody (Product # PA5-117017) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HIF1AN in L929 cells. Samples were incubated with HIF1AN Polyclonal antibody (Product # PA5-117017) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of HIF1AN in 200 μg extracts of 293T cells. Samples were precipitated with 1 μg HIF1AN Polyclonal antibody (Product # PA5-117017). Western blot was performed from the immunoprecipitate using HIF1AN Polyclonal antibody (Product # PA5-117017) at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of HIF1AN in 200 µg extracts of 293T cells using HIF1AN Polyclonal Antibody (Product # PA5-117017). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100