PA5-117714
antibody from Invitrogen Antibodies
Targeting: HIF1AN
DKFZp762F1811, FIH1, FLJ20615, FLJ22027
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-117714 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIF1AN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HIF1AN using HIF1AN Polyclonal Antibody (Product # PA5-117714) at 12 µg/mL. Lane 1: Mouse heart tissue. Lane 2: Mouse skeletal muscle tissue. Lane 3: K562 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: HL60 whole cell lysate. Lane 6: 293T whole cell lysate. Lane 7: Jurkat whole cell lysate. Lane 8: A375 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:10,000 dilution. Predicted band size: 41 kDa. Observed band size: 41, 48 kDa
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HIF1AN using HIF1AN Polyclonal Antibody (Product # PA5-117714) at 12 µg/mL. Lane 1: Mouse heart tissue. Lane 2: Mouse skeletal muscle tissue. Lane 3: K562 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: HL60 whole cell lysate. Lane 6: 293T whole cell lysate. Lane 7: Jurkat whole cell lysate. Lane 8: A375 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:10,000 dilution. Predicted band size: 41 kDa. Observed band size: 41, 48 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of HIF1AN in HeLa cells using HIF1AN Polyclonal Antibody (Product # PA5-117714) at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of HIF1AN in MCF-7 cells using HIF1AN Polyclonal Antibody (Product # PA5-117714) at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immnohistochemistry (Paraffin) analysis of HIF1AN in paraffin-embedded human kidney tissue using HIF1AN Polyclonal Antibody (Product # PA5-117714) diluted at 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating HIF1AN in MCF-7 whole cell lysate. Lane 1: Rabbit control IgG instead of (1 µg) MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: HIF1AN Polyclonal Antibody (Product # PA5-117714) (8 µg) + MCF-7 whole cell lysate (500 µg). Lane 3: MCF-7 whole cell lysate (10 µg).