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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-13125 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-13125, RRID:AB_671403
- Product name
- Anti-UBTF
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Las1L is a nucleolar protein required for cell proliferation and ribosome biogenesis.
CTCF regulates the local epigenetic state of ribosomal DNA repeats.
PHF8 activates transcription of rRNA genes through H3K4me3 binding and H3K9me1/2 demethylation
Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.
Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells.
Control of cell size through phosphorylation of upstream binding factor 1 by nuclear phosphatidylinositol 3-kinase.
Castle CD, Cassimere EK, Lee J, Denicourt C
Molecular and cellular biology 2010 Sep;30(18):4404-14
Molecular and cellular biology 2010 Sep;30(18):4404-14
CTCF regulates the local epigenetic state of ribosomal DNA repeats.
van de Nobelen S, Rosa-Garrido M, Leers J, Heath H, Soochit W, Joosen L, Jonkers I, Demmers J, van der Reijden M, Torrano V, Grosveld F, Delgado MD, Renkawitz R, Galjart N, Sleutels F
Epigenetics & chromatin 2010 Nov 8;3(1):19
Epigenetics & chromatin 2010 Nov 8;3(1):19
PHF8 activates transcription of rRNA genes through H3K4me3 binding and H3K9me1/2 demethylation
Weijun Feng, Masato Yonezawa, Jing Ye, Thomas Jenuwein, Ingrid Grummt
Nature Structural & Molecular Biology 2010 Mar;17(4):445-450
Nature Structural & Molecular Biology 2010 Mar;17(4):445-450
Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.
Sharov AA, Masui S, Sharova LV, Piao Y, Aiba K, Matoba R, Xin L, Niwa H, Ko MS
BMC genomics 2008 Jun 3;9:269
BMC genomics 2008 Jun 3;9:269
Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells.
Kopp K, Gasiorowski JZ, Chen D, Gilmore R, Norton JT, Wang C, Leary DJ, Chan EK, Dean DA, Huang S
Molecular biology of the cell 2007 Feb;18(2):394-403
Molecular biology of the cell 2007 Feb;18(2):394-403
Control of cell size through phosphorylation of upstream binding factor 1 by nuclear phosphatidylinositol 3-kinase.
Drakas R, Tu X, Baserga R
Proceedings of the National Academy of Sciences of the United States of America 2004 Jun 22;101(25):9272-6
Proceedings of the National Academy of Sciences of the United States of America 2004 Jun 22;101(25):9272-6
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 89
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction centra.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
