PA5-36153

antibody from Invitrogen Antibodies

Targeting: UBTF NOR-90, UBF, UBF1, UBF2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-36153

Provider

Invitrogen Antibodies

Product name

UBF-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 90 kDa. Purity is >95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Muscle from aged rats is resistant to mechanotherapy during atrophy and reloading.

Lawrence MM, Van Pelt DW, Confides AL, Hettinger ZR, Hunt ER, Reid JJ, Laurin JL, Peelor FF 3rd, Butterfield TA, Miller BF, Dupont-Versteegden EE
GeroScience 2021 Feb;43(1):65-83

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Massage as a mechanotherapy promotes skeletal muscle protein and ribosomal turnover but does not mitigate muscle atrophy during disuse in adult rats.

Lawrence MM, Van Pelt DW, Confides AL, Hunt ER, Hettinger ZR, Laurin JL, Reid JJ, Peelor FF 3rd, Butterfield TA, Dupont-Versteegden EE, Miller BF
Acta physiologica (Oxford, England) 2020 Jul;229(3):e13460

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UBF-1 in Lane 1: L02 whole cell lysate (40 µg), Lane 2: H9C2 whole cell lysate (20 µg), Lane 3: AML-12 whole cell lysate (20 µg), Lane 4: A549 whole cell lysate (20 µg). Samples were incubated with UBF-1 polyclonal antibody (Product # PA5-36153) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-UBF-1 Polyclonal Antibody (Product # PA5-36153) and ~110kDa band corresponding to UBF-1 was observed across the panel tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), HCT 116 (Lane 3), Jurkat (Lane 4) and tissue extracts (30ug lysate) of Mouse colon (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).