PA5-105512

antibody from Invitrogen Antibodies

Targeting: UBTF NOR-90, UBF, UBF1, UBF2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-105512

Provider

Invitrogen Antibodies

Product name

Phospho-UBF-1 (Thr117) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of UBF1 only when phosphorylated at Thr117.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-UBF-1 (Thr117) in HeLa cells treated with calyculinA (50 ng/mL) (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-UBF-1 (Thr117) in human gastric cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-UBF-1 (Thr117) in human lung cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-UBF-1 (Thr117) in mouse liver tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-UBF-1 (Thr117) in rat lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.