PA5-105512

antibody from Invitrogen Antibodies

Targeting: UBTF NOR-90, UBF, UBF1, UBF2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-105512

Provider

Invitrogen Antibodies

Product name

Phospho-UBF-1 (Thr117) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-UBF-1 (Thr117) in HeLa cells treated with calyculinA (50 ng/mL) (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-UBF-1 (Thr117) in human gastric cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-UBF-1 (Thr117) in human lung cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-UBF-1 (Thr117) in mouse liver tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-UBF-1 (Thr117) in rat lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-UBF-1 (Thr117) polyclonal antibody (Product # PA5-105512) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.