Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [14]
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Validation data
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- Product number
- NBP1-82545 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82545, RRID:AB_11032609
- Product name
- Rabbit Polyclonal UBTF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human UBTF antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase.
Loss of MYBBP1A Induces Cancer Stem Cell Activity in Renal Cancer.
UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity.
Venegas AB, Natsume T, Kanemaki M, Hickson ID
Cell reports 2020 Apr 21;31(3):107533
Cell reports 2020 Apr 21;31(3):107533
Loss of MYBBP1A Induces Cancer Stem Cell Activity in Renal Cancer.
Felipe-Abrio B, Verdugo-Sivianes EM, Sáez C, Carnero A
Cancers 2019 Feb 18;11(2)
Cancers 2019 Feb 18;11(2)
UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity.
Sobol M, Yildirim S, Philimonenko VV, Marášek P, Castaño E, Hozák P
Nucleus (Austin, Tex.) 2013 Nov-Dec;4(6):478-86
Nucleus (Austin, Tex.) 2013 Nov-Dec;4(6):478-86
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBTF Antibody [NBP1-82545] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBTF Antibody [NBP1-82545] - Analysis in mouse cerebral cortex tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBTF Antibody [NBP1-82545] - Analysis in human cell line MOLT-4.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBTF Antibody [NBP1-82545] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: UBTF Antibody [NBP1-82545] - Staining of mouse thalamus shows nuclear immunoreactivity in anterodorsal thalamic nucleus neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human cerebral cortex shows nuclear and cytoplasmic immunoreactivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: UBTF Antibody [NBP1-82545] - Staining of mouse retrosplenial granular cortex shows nuclear neuronal positivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human skin shows strong nuclear positivity in epidermal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human hippocampus shows nuclear immunoreactivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: UBTF Antibody [NBP1-82545] - Staining of mouse motor cortex shows nuclear positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human cerebral cortex shows nuclear positivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of mouse basal forebrain shows moderate nuclear positivity in neurons in the caudate putamen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of mouse brain shows strong nuclear positivity in neurons in the cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of mouse brain shows strong positivity in neurons in the CA1 and granular cell layers in the hippocampus.