Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
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- Product number
- PA5-70400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP H1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP-H1 on human MCF7 cells. The sample was probed with a hnRNP-H1 polyclonal antibody (Product # PA5-70400) using a primary antibody dilution of 1.0 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP-H1 on HepG2 cells. The sample was probed with a hnRNP-H1 polyclonal antibody (Product # PA5-70400) using a primary antibody dilution of 0.2-1.0 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of hnRNP H1 was achieved by transfecting Hep G2 with hnRNP H1 specific siRNAs (Silencer® select Product # s6728, s6729). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1%SDS) from the knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with hnRNP H1 Polyclonal Antibody (Product # PA5-70400, 0.5 µg/mL) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to hnRNP H1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-hnRNP H1 Polyclonal Antibody, (Product # PA5-70400) and a 50 kDa band corresponding to hnRNP H1 was observed in the cell lines tested. Modified whole cell extracts (1%SDS) (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), BeWo (Lane 3), Raji (Lane 4) and Jurkat (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of hnRNP H1 in MCF-7 cells. Samples were treated with hnRNP H1 polyclonal antibody (Product # PA5-70400) with a dilution of 1:200, a secondary antibody using anti-rabbit FITC with a dilution of 1:500 and DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry was performed on MCF-7 tissue using a hnRNP H1 polyclonal primary antibody (Product # PA5-70400) with a dilution of 1:200, anti-rabbit FITC with a secondary antibody dilution of 1:500 and DAPI (Blue) and HNRPH1 (Green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HNRNPH1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with hnRNP H1 Polyclonal Antibody (Product # PA5-70400) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing staining in nucleus. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.