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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A300-511A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP-H Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse hnRNP-H by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP-H antibody (Product # A300-511A; lot A300-511A-2) used for WB at 0.2 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse hnRNP H by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP H antibody A300-511A (lot A300-511A-2) used for WB at 0.2 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. hnRNP H was also immunoprecipitated by a prior lot (lot A300-511A-1) of this antibody. Detection: Chemiluminescence with exposure times 30 second (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human hnRNP H by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- hnRNP H (Cat. No. A300-511A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human hnRNP-H by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-hnRNP-H antibody (Product # A300-511A; lot A300-511A-2) used for WB at 0.2 µg/mL. hnRNP-H was also immunoprecipitated by a previous lot (A300-511A-1) of this antibody. For blotting immunoprecipitated hnRNP-H (Product # A300-511A) was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
