NB100-385

Lee KY, Li M, Manchanda M, Batra R, Charizanis K, Mohan A, Warren SA, Chamberlain CM, Finn D, Hong H, Ashraf H, Kasahara H, Ranum LP, Swanson MS
EMBO molecular medicine 2013 Dec;5(12):1887-900

G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site.

Wang E, Mueller WF, Hertel KJ, Cambi F
The Journal of biological chemistry 2011 Feb 11;286(6):4059-71

A glycine-rich domain of hnRNP H/F promotes nucleocytoplasmic shuttling and nuclear import through an interaction with transportin 1.

Van Dusen CM, Yee L, McNally LM, McNally MT
Molecular and cellular biology 2010 May;30(10):2552-62

Regulation of alternative splicing of the receptor for advanced glycation endproducts (RAGE) through G-rich cis-elements and heterogenous nuclear ribonucleoprotein H.

Ohe K, Watanabe T, Harada S, Munesue S, Yamamoto Y, Yonekura H, Yamamoto H
Journal of biochemistry 2010 May;147(5):651-9

ALS-associated mutations in TDP-43 increase its stability and promote TDP-43 complexes with FUS/TLS.

Ling SC, Albuquerque CP, Han JS, Lagier-Tourenne C, Tokunaga S, Zhou H, Cleveland DW
Proceedings of the National Academy of Sciences of the United States of America 2010 Jul 27;107(30):13318-23

Biochemical and genetic evidence for a role of IGHMBP2 in the translational machinery.

de Planell-Saguer M, Schroeder DG, Rodicio MC, Cox GA, Mourelatos Z
Human molecular genetics 2009 Jun 15;18(12):2115-26

Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs.

Wang E, Cambi F
The Journal of biological chemistry 2009 Apr 24;284(17):11194-204

Molecular composition of IMP1 ribonucleoprotein granules.

Jønson L, Vikesaa J, Krogh A, Nielsen LK, Hansen Tv, Borup R, Johnsen AH, Christiansen J, Nielsen FC
Molecular & cellular proteomics : MCP 2007 May;6(5):798-811

Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study.

Zhang D, Tai YC, Wong CH, Tai LK, Koay ES, Chen CS
Leukemia research 2007 Jan;31(1):75-82

Identification of proteins cleaved downstream of caspase activation in monocytes undergoing macrophage differentiation.

Cathelin S, Rébé C, Haddaoui L, Simioni N, Verdier F, Fontenay M, Launay S, Mayeux P, Solary E
The Journal of biological chemistry 2006 Jun 30;281(26):17779-88

Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.

McNally LM, Yee L, McNally MT
The Journal of biological chemistry 2006 Feb 3;281(5):2478-88

Allogenic antibody-mediated identification of head and neck cancer antigens.

Rauch J, Ahlemann M, Schaffrik M, Mack B, Ertongur S, Andratschke M, Zeidler R, Lang S, Gires O
Biochemical and biophysical research communications 2004 Oct 8;323(1):156-62

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: hnRNP H Antibody [NB100-385] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP H antibody NB100-385 (lot 2) used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. hnRNP H was also immunoprecipitated by a prior lot (lot 1) of this antibody. Detection: Chemiluminescence with exposure times 30 second (A and B).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: hnRNP H Antibody [NB100-385] - Simple Western lane view shows a specific band for hnRNP H in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-hnRNP-H used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-hnRNP-H at dilution of 1:250. DAB staining using anti-Rabbit IHC antibody at dilution of 1:100.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- hnRNP H used at a dilution of 1:1,000 (1ug/ml). Detection: DAB