PAB27498

antibody from Abnova Corporation

Targeting: ZNF227

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27498

Provider

Abnova Corporation

Product name

ZNF227 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ZNF227.

Antigen sequence

FWRTQHSCGNTYLSESQIQSRGKQIDVKNNLQIHE
DFMKKSPFHEHIKTDTEPKPCKGNEYGKIISDGSN
QKLPL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with ZNF227 polyclonal antibody (Cat # PAB27498) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with ZNF227 polyclonal antibody (Cat # PAB27498) at 1-4 ug/mL dilutions shows positivity in plasma membrane.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human spleen with ZNF227 polyclonal antibody (Cat # PAB27498) shows strong cytoplasmic positivity in subsets of cells in red pulp at 1:500-1:100 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)