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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-41972 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FZD4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: GITSGMWIWS AKTLHTWQKC SNRLVNSGKV KREKRGNGWV KPGKGSETVV Sequence homology: Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Single-Cell Transcriptional Profiling Reveals Sex and Age Diversity of Gene Expression in Mouse Endothelial Cells.
Huang X, Shen W, Veizades S, Liang G, Sayed N, Nguyen PK
Frontiers in genetics 2021;12:590377
Frontiers in genetics 2021;12:590377
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 10 Expression of selected genes and proteins in aortic ECs. (A) Bar graphs displaying upregulation of the expression of Lars2 as well as genes involved in the Wnt pathway at 3 months (top) and upregulation of S100A8 and S100A9 at 18-months in males relative to females by quantitative PCR (bottom). (B) Western blot analysis confirming the expression of Lars2 as well as genes involved in the Wnt pathway are expressed at the protein level (top) and displayed as relative fold-difference between males and females in a bar graph (bottom). *** P < 0.001; ** P < 0.01; and * P < 0.01.
