LS-B10900

antibody from LSBio

Targeting: HTATIP2 CC3, FLJ26963, SDR44U1, TIP30

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B10900

Provider

LSBio

Product name

IHC-plus™ TIP30 / HTATIP2 Antibody LS-B10900

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: A431 B: HeLa 12% SDS PAGE HTATIP2 / TIP30 antibody diluted at 1:5000

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IP Sample: hela whole cell lysate/extract A. 40 µg hela whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 µg of TIP30 antibody 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody diluted at 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IP Sample: hela whole cell lysate/extract A. 40 µg hela whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 µg of TIP30 antibody 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody diluted at 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IP Sample: hela whole cell lysate/extract A. 40 µg hela whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 µg of TIP30 antibody 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody diluted at 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-TIP30 / HTATIP2 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.