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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX100119 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100119, RRID:AB_11165752
- Product name
- TIP30 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 B: HeLa 12% SDS PAGE GTX100119 diluted at 1:5000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TIP30(GTX100119) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TIP30 antibody immunoprecipitates TIP30 protein in IP experiments. IP Sample: hela whole cell lysate/extract A. 40 £gg hela whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 £gg of TIP30 antibody (GTX100119) 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody (GTX100119) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using TIP30(GTX100119) antibody at 1:250 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TIP30 antibody detects TIP30 protein on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. TIP30 antibody (GTX100119) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TIP30 antibody detects TIP30 protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. TIP30 antibody (GTX100119) diluted at 1:500.
