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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-27109 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIP30 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: A431, HeLa. Predicted reactivity: Mouse (90%), Rat (88%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TIP30 in paraformaldehyde-fixed HeLa cells using a TIP30 polyclonal antibody (Product # PA5-27109) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TIP30 Polyclonal Antibody immunoprecipitates TIP30 protein in IP experiments. IP Sample: hela whole cell lysate/extract A. 40 µg hela whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 µg of TIP30 Polyclonal Antibody (Product # PA5-27109) 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 Polyclonal Antibody (Product # PA5-27109) diluted at 1:1,000. Anti-rabbit IgG was used as a secondary reagent.
