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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PA5-87462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ENDOG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AELPPVPGGP RGPGELAKYG LPGLAQLKSR ESYVLCYDPR TRGALWVVEQ LRPERLRGDG DRRECDFRED DSVHAYHRAT NADYRGSGFD RGHLAAAANH RWSQKAMDDT FYLSNVAPQV PHLNQNAWNN LEKYSRSLTR SYQNVYVCTG PLFLPRTEAD GKSYVKYQVI GKNHVAVPTH FFKVLILEAA GGQIELRTYV MPNAPVDEAI PLERFLVPIE SIERASGLLF VPNILARAGS LKAITAGSK; Positive Samples: HeLa, MCF7, SW620, Mouse heart, Mouse kidney, Rat heart; Cellular Location: Mitochondrion
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.99 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ENDOG in extracts of various cell lines using ENDOG Polyclonal Antibody (Product # PA5-87462) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ENDOG in paraffin-embedded mouse kidney using ENDOG Polyclonal Antibody (Product # PA5-87462) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ENDOG in paraffin-embedded mouse lung using ENDOG Polyclonal Antibody (Product # PA5-87462) at a dilution of 1:100.
