PA5-20004

antibody from Invitrogen Antibodies

Targeting: ENDOG

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20004

Provider

Invitrogen Antibodies

Product name

ENDOG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In Western blot applications, this antibody detects a band at ~35kDa. A suggested positive control is HepG2 cell lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse (M) 3T3 and human (H) HepG2 cell lysates using a EndoG polyclonal antibody (Product # PA5-20004) at 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human A431 Cell Lysate with the presence (A) or absence (B and C) of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: ENDOG Polyclonal Antibody (Product # PA5-20004) (A: 0.5 µg/mL, B: 0.5 µg/mL, C: 1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Mouse 3T3 (M) and Human HepG2 (H) Cell Lysates. Loading: 15 µg of lysates per lane. Antibodies: ENDOG Polyclonal Antibody (Product # PA5-20004) (2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human EndoG recombinant protein per lane. Antibodies: ENDOG Polyclonal Antibody (Product # PA5-20004) 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 0.5 µg/mL Lane 2: 1 µg/mL Lane 3: 2 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling EndoG with ENDOG Polyclonal Antibody (Product # PA5-20004) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using ENDOG Polyclonal Antibody (Product # PA5-20004) at 15 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using ENDOG Polyclonal Antibody (Product # PA5-20004) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.