HPA021323

antibody from Atlas Antibodies

Targeting: GCC1 FLJ22035, GCC1P, GCC88, MGC20706

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA021323

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA021323, RRID:AB_1849554

Product name

Anti-GCC1

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

GDPADTSSSDSLTQALQLAAANEPTFFLYAEQLAR
KEVEITSLRKQKHRLEVEVHQLQDRLLEEGERHRE
EVAALQSHIEKNIRDQSREGANLEYLKNIIYRFLT
LPDSLGRQQTLTAILTILHFSPEEKQVIMRLPT


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins

Wong M, Munro S
Science 2014 October;346(6209):1256898-1256898

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GCC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human colon, kidney, liver and testis using Anti-GCC1 antibody HPA021323 (A) shows similar protein distribution across tissues to independent antibody HPA019369 (B).

Antibody #2 product nr

HPA019369

Antibody provider

Atlas Antibodies

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver using Anti-GCC1 antibody HPA021323.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-GCC1 antibody HPA021323.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney using Anti-GCC1 antibody HPA021323.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-GCC1 antibody HPA021323.

Sample type

HUMAN