PA5-54381

antibody from Invitrogen Antibodies

Targeting: GCC1 FLJ22035, GCC1P, GCC88, MGC20706

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54381

Provider

Invitrogen Antibodies

Product name

GCC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GDPADTSSSD SLTQALQLAA ANEPTFFLYA EQLARKEVEI TSLRKQKHRL EVEVHQLQDR LLEEGERHRE EVAALQSHIE KNIRDQSREG ANLEYLKNII YRFLTLPDSL GRQQTLTAIL TILHFSPEEK QVIMRLPT Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GCC1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a GCC1 Polyclonal Antibody (Product # PA5-54381).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GCC1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a GCC1 Polyclonal Antibody (Product # PA5-54381).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of GCC1 in human cell line A-431 shows positivity in the Golgi apparatus. Samples were probed using a GCC1 Polyclonal Antibody (Product # PA5-54381).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GCC1 in human kidney using GCC1 Polyclonal Antibody (Product # PA5-54381).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GCC1 in human colon using GCC1 Polyclonal Antibody (Product # PA5-54381).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GCC1 in human testis using GCC1 Polyclonal Antibody (Product # PA5-54381).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GCC1 in human liver using GCC1 Polyclonal Antibody (Product # PA5-54381).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of GCC1 in human colon, kidney, liver and testis using GCC1 Polyclonal Antibody (Product # PA5-54381) (A) shows similar protein distribution across tissues to an independent GCC1 Polyclonal Antibody (B).