NBP1-83609

antibody from Novus Biologicals

Targeting: GCC1 FLJ22035, GCC1P, GCC88, MGC20706

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-83609

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-83609, RRID:AB_11015326

Product name

Rabbit Polyclonal GCC1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of GCC1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat, Canine

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Membrane trafficking. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins.

Wong M, Munro S
Science (New York, N.Y.) 2014 Oct 31;346(6209):1256898

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GCC1 Antibody [NBP1-83609] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GCC1 Antibody [NBP1-83609] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GCC1 Antibody [NBP1-83609] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GCC1 Antibody [NBP1-83609] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP1-83609] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP1-83609] - Staining of human colon, kidney, liver and testis using Anti-GCC1 antibody NBP1-83609 (A) shows similar protein distribution across tissues to independent antibody NBP1-83610 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP1-83609] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP1-83609] - Staining of human liver.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP1-83609] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GCC1 Antibody [NBP1-83609] - Staining of human kidney.