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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB2746 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2746, RRID:AB_10549747
- Product name
- APAF1 monoclonal antibody, clone 2E10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide of APAF1.
- Isotype
- IgG
- Antibody clone number
- 2E10
- Storage
- Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development.
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation.
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade.
Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P
Cell 1998 Sep 18;94(6):727-37
Cell 1998 Sep 18;94(6):727-37
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation.
Hu Y, Benedict MA, Wu D, Inohara N, Núñez G
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 14;95(8):4386-91
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 14;95(8):4386-91
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade.
Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X
Cell 1997 Nov 14;91(4):479-89
Cell 1997 Nov 14;91(4):479-89
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of APAF1 in K-562 cell lysate with APAF1 monoclonal antibody, clone 2E10 (Cat # MAB2746) at (A) 0.25 and (B) 0.5 mg/ml.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry of APAF1 in K-562 cells with APAF1 monoclonal antibody, clone 2E10 (Cat # MAB2746) at 0.5 ug/mL .
- Validation comment
- Immunocytochemistry
