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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [3]
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Validation data
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- Product number
- AM1194A - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1194a, RRID:AB_2170131
- Product name
- PRDM2 (RIZ1) Antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Purified Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1.25 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references RIZ1 is potential CML tumor suppressor that is down-regulated during disease progression.
A histone methyltransferase is required for maximal response to female sex hormones.
Lakshmikuttyamma A, Takahashi N, Pastural E, Torlakovic E, Amin HM, Garcia-Manero G, Voralia M, Czader M, DeCoteau JF, Geyer CR
Journal of hematology & oncology 2009 Jul 14;2:28
Journal of hematology & oncology 2009 Jul 14;2:28
A histone methyltransferase is required for maximal response to female sex hormones.
Carling T, Kim KC, Yang XH, Gu J, Zhang XK, Huang S
Molecular and cellular biology 2004 Aug;24(16):7032-42
Molecular and cellular biology 2004 Aug;24(16):7032-42
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PRDM2 Mab (Cat. #AM1194a) in lysate from PRDM2 transgenic lysate.Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using anti-RIZ1 antibody (AM1194a). Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in Carling et al., 2004 (see citation 2)."
- Primary Ab dilution
- 1:100
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "ChIP analysis of estrogen target genes.(B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with antibody to RIZ1 (AM1194a) and control immunoglobulin G as indicated. Adapted from Fig 6 in Carling et al., 2004 (see citation 2)."
- Primary Ab dilution
- 1:100
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibody against RIZ1 (AM1194a). DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in Carling et al., 2004 (see citation 2)."
- Primary Ab dilution
- 1:100
