Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [6]
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- Product number
- PA5-72816 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOX4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Hyperoxaluria Induces Endothelial Dysfunction in Preglomerular Arteries: Involvement of Oxidative Stress.
Acute glucose fluctuation promotes in vitro intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway.
Rapamycin Suppresses Penile NADPH Oxidase Activity to Preserve Erectile Function in Mice Fed a Western Diet.
Chalcone suppresses tumor growth through NOX4-IRE1α sulfonation-RIDD-miR-23b axis.
ROS production and mitochondrial dysfunction driven by PU.1-regulated NOX4-p22(phox) activation in Aβ-induced retinal pigment epithelial cell injury.
Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.
Saenz-Medina J, Muñoz M, Rodriguez C, Contreras C, Sánchez A, Coronado MJ, Ramil E, Santos M, Carballido J, Prieto D
Cells 2022 Jul 27;11(15)
Cells 2022 Jul 27;11(15)
Acute glucose fluctuation promotes in vitro intestinal epithelial cell apoptosis and inflammation via the NOX4/ROS/JAK/STAT3 signaling pathway.
Chen B, Jia Y, Lu D, Sun Z
Experimental and therapeutic medicine 2021 Jul;22(1):688
Experimental and therapeutic medicine 2021 Jul;22(1):688
Rapamycin Suppresses Penile NADPH Oxidase Activity to Preserve Erectile Function in Mice Fed a Western Diet.
La Favor JD, Pierre CJ, Bivalacqua TJ, Burnett AL
Biomedicines 2021 Dec 30;10(1)
Biomedicines 2021 Dec 30;10(1)
Chalcone suppresses tumor growth through NOX4-IRE1α sulfonation-RIDD-miR-23b axis.
Kim HK, Lee HY, Riaz TA, Bhattarai KR, Chaudhary M, Ahn JH, Jeong J, Kim HR, Chae HJ
Redox biology 2021 Apr;40:101853
Redox biology 2021 Apr;40:101853
ROS production and mitochondrial dysfunction driven by PU.1-regulated NOX4-p22(phox) activation in Aβ-induced retinal pigment epithelial cell injury.
Sun J, Chen J, Li T, Huang P, Li J, Shen M, Gao M, Sun Y, Liang J, Li X, Wang Y, Xiao Y, Shi X, Hu Y, Feng J, Jia H, Liu T, Sun X
Theranostics 2020;10(25):11637-11655
Theranostics 2020;10(25):11637-11655
Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.
Waddingham MT, Sonobe T, Tsuchimochi H, Edgley AJ, Sukumaran V, Chen YC, Hansra SS, Schwenke DO, Umetani K, Aoyama K, Yagi N, Kelly DJ, Gaderi S, Herwig M, Kolijn D, Mügge A, Paulus WJ, Ogo T, Shirai M, Hamdani N, Pearson JT
Journal of molecular and cellular cardiology 2019 Dec;137:119-131
Journal of molecular and cellular cardiology 2019 Dec;137:119-131
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NOX4 in Jurkat cell lysate with NOX4 Polyclonal Antibody (Product # PA5-72816) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of NOX4 in human spleen tissue with NOX4 Polyclonal Antibody (Product # PA5-72816) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of NOX4 in human spleen tissue with NOX4 Polyclonal Antibody (Product # PA5-72816) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 PU.1 upregulation is concomitant with NOX4- p22 phox complex synthesis. ( A ) PU.1, p22 phox (CYBA), and NOX4 expression and quantification in mouse RPE cells after 2, 4, and 6 days of Abeta 1-40 treatment. ( B ) Co-IP of NOX4 and p22 phox (CYBA) at 4 days after the injection of Abeta 1-40 or PBS. ( C, D ) Retinal sections were immuno-stained for NOX4 (C) and p22 phox (D) at 4 days post-injection. The lower parts of the images are magnified portions of the RPE layer from the upper parts of the images (white boxes). Scale bar = 50 um; all data are presented as the mean +- SEM; n = 3 for each group. NS = nonsignificant, *** P < 0.001, Student's t -test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 NOX4 expression in intestinal epithelial cells among the various treatment groups. (A) NOX4 protein expression levels in intestinal epithelial cells were assessed using western blot analysis. (B) Representative immunofluorescence staining images of NOX4 expression (green) in intestinal epithelial cells; nuclei were stained with DAPI (blue); magnification, x400. ** P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Degradation of miR-23b by IRE1alpha-RIDD increased NOX4 expression in ER. (A) Immunoblotting of NOX family was performed using cell lysates treated with different concentrations of chalcone in MDA-MB-231 and PC-3. (B) Immunoblotting of NOX4 was performed in subcellular fractionation of PC-3 cells treated with chalcone. (C) NOX activity was measured by chemiluminescence (n = 3 independent experiments). (D) Live cells was confirmed in PC-3 cells with NOX4 siRNA transfection compared with cells transfected with scrambled siRNA oligonucleotides (n = 3 independent experiments). Right; immunoblotting was performed to validate knockdown of NOX4. (E) Immunoprecipitation using anti-sulfonate antibody was performed on MDA-MB-231 cells with NOX4 siRNA, scrambled siRNA upon the treatment of chalcone. (F-G) NOX4 expression was analyzed using the Gene Expression Omnibus database from NCBI. Prostate (GSE3325, F), and Breast (GSE31448, G) datasets are presented. (H) Representative immunohistochemical staining of NOX4 on tissue microarrays. Right; quantification data of NOX4 expression. Scale bar, 100 mum. (brown: positive antibody staining, blue: hematoxylin for nuclear staining). (I) The mRNA expression of NOX4 was measured by qRT-PCR (n = 3 independent experiments). (J) Sequence motif of miR-23b-3p binding sites to NOX4. (K) miR-23b-3p expression was detected after treatment of chalcone (n = 3 independent experiments). (L) Sequence motif for the IRE1alpha cleavage sites (upper) and
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Chalcone activated NOX4-IRE1alpha sulfonation-RIDD axis in vivo . (A) DHE staining in tumor samples from generating xenograft (XG) mouse model with or without chalcone treatment. Right; quantification data of DHE intensity (n = 5 mice per group). Scale bar, 25 mum. (B) ER lipid peroxidation was measured in xenograft tumors (n = 5 mice per group). (C) PLA assay between IRE1alpha and sulfonation (red dot) in xenograft tumors (n = 5 mice per group). Right; quantification data of red dots (n = 5 mice per group). Scale bar, 25 mum. (D) Immunoprecipitation using anti-sulfonate antibody was performed in xenograft tumors. (E) The mRNA expression of splicing XBP1 were measured by qRT-PCR (n = 5 mice per group). (F) Heatmap of the mRNA expression of the indicated genes as RIDD substrates represented (n = 5 mice per group). (G) Immunoblotting of NOX4 was performed using cell lysates from xenograft tumors. (H-I) The mRNA expression of NOX4 (H) and miR-23b-3p (I) were measured by qRT-PCR (n = 5 mice per group). Data represent mean +- SD. Statistical differences was detected with two-way ANOVA followed by Bonferroni's test. Asterisks indicate significant differences from the control. Cha; chalcone. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) Fig. 5
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- Figure 4 Assessment of NADPH oxidase subunit protein content in the corpus cavernosum. Mice were fed the control (CD) or Western (WD) diet and treated with vehicle or 2 mg/kg rapamycin (Rap). ( A ) Representative immunoblot images for the NADPH oxidase subunits and the loading control GAPDH. Quantification of band intensity density normalized to GAPDH for: ( B ) gp91 phox ; ( C ) p67 phox ; ( D ) p47 phox ; ( E ) p22 phox ; ( F ) Nox4. Data are presented as mean +- standard error of the mean for n = 8-9 mice per group; * p < 0.05 vs. CD-Veh, ++ p < 0.05 vs. WD-Veh.
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- Experimental details
- Unchanged Nox4 mRNA levels in preglomerular arteries from hyperoxaluric rats. ( A ) Comparative Nox4 mRNA expression in samples of renal arteries from the control ( n = 5) and OX group ( n = 7), performed by RT-PCR. The delta CT method was used to determine the fold change for NOX4 gene relative expression in the arteries that were treated with water (control group) and with 0.75% EG (OX group). Values represent the mean +- SD. Statistical differences were calculated by a Student's t -test for unpaired observations. No significant differences were found. ( B ) Immunofluorescence demonstration of NOX4 protein in a section of a renal interlobar artery from the Control and OX group. Immunofluorescence double labeling for TO-PRO marker (blue areas) demonstrates nuclear staining and for NOX4 protein (red areas).