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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- RQ4166 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NADPH oxidase 4 Antibody / NOX4
- Antibody type
- Polyclonal
- Description
- This highly specific NADPH oxidase 4 antibody is suitable for use in Immunofluorescence/Western blot applications with human, monkey samples.
- Reactivity
- Human, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NADPH oxidase 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat kidney, 2) rat NRK, 3) mouse kidney, 4) mouse HEPA1-6 and 5) mouse SP20 lysate with NADPH oxidase 4 antibody at 0.5ug/ml. Expected molecular weight: ~65 kDa, 75-80 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human 293T, 2) human U-87 MG, 3) human SH-SY5Y, 4) human U-251, 5) human U-2 OS, 6) human HeLa, 7) human T-47D and 8) monkey COS-7 cell lysate with NADPH oxidase 4 antibody. Expected molecular weight: ~65 kDa, 75-80 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with NADPH oxidase 4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human renal cancer tissue with NADPH oxidase 4 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
