Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C782085 - Provider product page
- Provider
- LSBio
- Product name
- NOX4 Antibody LS-C782085
- Antibody type
- Polyclonal
- Description
- Antigen Affinity purification
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- After reconstitution, store at 4°C for up to 1 month. Long-term: aliquot and store at -20°C. Avoid freeze-thaws cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot testing of 1) rat kidney, 2) rat NRK, 3) mouse kidney, 4) mouse HEPA1-6 and 5) mouse SP20 lysate with NADPH oxidase 4 antibody at 0.5ug/ml. Expected molecular weight: ~65 kDa, 75-80 kDa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2, 4) A549 and 5) 293T cell lysate with NADPH oxidase 4 antibody at 0.5ug/ml. Expected molecular weight: ~65 kDa, 75-80 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC testing of FFPE human renal cancer tissue with NADPH oxidase 4 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.