Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-80374 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCAM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCAM in Lane A: A431 Whole Cell Lysate (30 µg). Samples were probed using a BCAM Polyclonal Antibody (Product # PA5-80374) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:67 kDa. Observed band size:90 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BCAM Polyclonal Antibody (Product # PA5-80374) at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 67 kDa. Observed band size: 90 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human BCAM in human breast carcinoma with BCAM Polyclonal Antibody (Product # PA5-80374, 1:1,000, formalin-fixed paraffin embedded sections). Positive staining was localized to the epithelia and endothelium.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human BCAM in human tonsil with BCAM Polyclonal Antibody (Product # PA5-80374, 1:1,000, formalin-fixed paraffin embedded sections). Positive staining was localized to the endothelium of blood vessel walls.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCAM Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg MCF-7 Whole Cell Lysate 2 µL with BCAM Polyclonal Antibody (Product # PA5-80374) and 15 µL of 50 % Protein G agarose. Primary antibody: BCAM Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 90 kDa. Observed band size: 90 kDa.