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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-22103 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBM28 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HepG2, Raji. Predicted reactivity: Mouse (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RBM28 Polyclonal Antibody (Product # PA5-22103). Sample (30 µg of whole cell lysate). Lane A: 293T . 7.5% SDS PAGE. RBM28 Polyclonal Antibody (Product # PA5-22103) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using RBM28 antibody (Product # PA5-22103) (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2500.
