935201

antibody from BioLegend

Targeting: IMPDH1 LCA11, RP10, sWSS2608

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

935201

Provider

BioLegend

Product name

Purified anti-IMPDH1

Antibody type

Monoclonal

Reactivity

Human, Mouse, Hamster

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

1D6-E3

Vial size

25 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-IMPDH1 antibody (clone 1D6-E3) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.

Conjugate

Unconjugated