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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-27792 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IMPDH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (95%), Rat (82%), Xenopus laevis (88%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Target identification reveals protein arginine methyltransferase 1 is a potential target of phenyl vinyl sulfone and its derivatives.
Yu CH, Chou CC, Lee DY, Khoo KH, Chang GD
Bioscience reports 2018 Apr 26;38(2)
Bioscience reports 2018 Apr 26;38(2)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 6 Confirmation of PVS and PVSN tagging by immunoprecipitation followed by immunoblotting HeLa cells were treated with 500 muM PVS or PVSN in PBS for 5 min and the lysate was subjected to anti-PVS immunoprecipitation. The immunoprecipitate was then probed with anti-protein arginine methyltransferase 1 (PRMT1), anti-glutathione reductase (GR), and anti-vimentin (upper panel). For inosine-5'-monophosphate dehydrogenase 1 (IMPDH1), the lysate was immunoprecipitated with anti-IMPDH1 and then the immunoprecipitate was probed with anti-PVS (lower panel). The control groups were treated with the same volume of DMSO.
