12573-1-AP

antibody from Proteintech Group

Targeting: TRIM25 EFP, RNF147, ZNF147

Provider product page for 12573-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [29]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

Submit

Product number: 12573-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12573-1-AP, RRID:AB_2209732
Product name: TRIM25 antibody
Antibody type: Polyclonal
Description: KD/KO validated TRIM25 antibody (Cat. #12573-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

TRIM25 protein structure - 12573-1-AP shown in red.

Submitted references Noncoding RNA Terc-53 and hyaluronan receptor Hmmr regulate ageing in mice.

Wu S, Cai Y, Zhang L, Li X, Liu X, Zhou G, Luo H, Li R, Huo Y, Zhang Z, Chen S, Huang J, Shi J, Ding S, Sun Z, Zhou Z, Wang P, Wang G
Protein & cell 2024 May 9;

TRIM25 predominately associates with anti-viral stress granules.

Shang Z, Zhang S, Wang J, Zhou L, Zhang X, Billadeau DD, Yang P, Zhang L, Zhou F, Bai P, Jia D
Nature communications 2024 May 15;15(1):4127

Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis.

Yan Y, Zhou S, Chen X, Yi Q, Feng S, Zhao Z, Liu Y, Liang Q, Xu Z, Li Z, Sun L
Signal transduction and targeted therapy 2024 Mar 4;9(1):58

Housekeeping U1 snRNA facilitates antiviral innate immunity by promoting TRIM25-mediated RIG-I activation.

Zhang F, Liu S, Qiao Z, Li L, Han Y, Sun J, Ge C, Zhu J, Li D, Yao H, Zhang H, Dai J, Yan Y, Chen Z, Yin L, Ma F
Cell reports 2024 Mar 26;43(3):113945

The long noncoding RNA HNF1A-AS1 with dual functions in the regulation of cytochrome P450 3A4.

Wang Y, Wang P, Wang Q, Chen S, Wang X, Zhong X, Hu W, Thorne RF, Han S, Wu M, Zhang L
Biochemical pharmacology 2024 Feb;220:116016

TRIM38 Induced in Respiratory Syncytial Virus-infected Cells Downregulates Type I Interferon Expression by Competing with TRIM25 to Bind RIG-I.

Sun Q, Han X, Meng L, Li H, Chen Y, Yin L, Wang C, Wang J, Li M, Gao X, Li W, Wei L, Ma C
Inflammation 2024 Aug;47(4):1328-1343

TRIM25-mediated ubiquitination of G3BP1 regulates the proliferation and migration of human neuroblastoma cells.

Yang Y, Luo Y, Yang C, Hu R, Qin X, Li C
Biochimica et biophysica acta. Gene regulatory mechanisms 2023 Sep;1866(3):194954

LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter.

Ren G, Li H, Hong D, Hu F, Jin R, Wu S, Sun W, Jin H, Zhao L, Zhang X, Liu D, Huang C, Huang H
BMC cancer 2023 Sep 23;23(1):898

Derlin-1 ameliorates nonalcoholic hepatic steatosis by promoting ubiquitylation and degradation of FABP1.

You H, Wen X, Wang X, Zhu C, Chen H, Bu L, Zhang J, Qu S
Free radical biology & medicine 2023 Oct;207:260-271

p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP.

Huang Y, Xiong C, Wang C, Deng J, Zuo Z, Wu H, Xiong J, Wu X, Lu H, Hao Q, Zhou X
Cell reports 2023 Nov 28;42(11):113426

Regulation of STING activity in DNA sensing by ISG15 modification.

Lin C, Kuffour EO, Fuchs NV, Gertzen CGW, Kaiser J, Hirschenberger M, Tang X, Xu HC, Michel O, Tao R, Haase A, Martin U, Kurz T, Drexler I, Görg B, Lang PA, Luedde T, Sparrer KMJ, Gohlke H, König R, Münk C
Cell reports 2023 Nov 28;42(11):113277

PRMT5 reduces immunotherapy efficacy in triple-negative breast cancer by methylating KEAP1 and inhibiting ferroptosis.

Wang Z, Li R, Hou N, Zhang J, Wang T, Fan P, Ji C, Zhang B, Liu L, Wang Y, Kong J, Yao Q, Duan J, Zhao G, Ling R, Zhang J
Journal for immunotherapy of cancer 2023 Jun;11(6)

EETs alleviate alveolar epithelial cell senescence by inhibiting endoplasmic reticulum stress through the Trim25/Keap1/Nrf2 axis.

Zhang CY, Zhong WJ, Liu YB, Duan JX, Jiang N, Yang HH, Ma SC, Jin L, Hong JR, Zhou Y, Guan CX
Redox biology 2023 Jul;63:102765

The E3 ubiquitin ligase MIB1 suppresses breast cancer cell migration through regulating CTNND1 protein level.

Kanoh T, Lu J, Mizoguchi T, Itoh M
Biochemical and biophysical research communications 2023 Jul 30;667:73-80

JP1, a polypeptide specifically targeting integrin αVβ3, ameliorates choroidal neovascularization and diabetic retinopathy in mice.

Xie Z, Wu XJ, Cheng RW, Cui JH, Yuan ST, Zhou JW, Liu QH
Acta pharmacologica Sinica 2023 Apr;44(4):897-912

Small Nucleolar RNA and C/D Box 15B Regulate the TRIM25/P53 Complex to Promote the Development of Endometrial Cancer.

Wen JT, Chen X, Liu X, Xie BM, Chen JW, Qin HL, Zhao Y
Journal of oncology 2022;2022:7762708

RITA1 drives the growth of bladder cancer cells by recruiting TRIM25 to facilitate the proteasomal degradation of RBPJ.

Tang H, Li X, Jiang L, Liu Z, Chen L, Chen J, Deng M, Zhou F, Zheng X, Liu Z
Cancer science 2022 Sep;113(9):3071-3084

Atypical E3 ligase ZFP91 promotes small-molecule-induced E2F2 transcription factor degradation for cancer therapy.

Liu TT, Yang H, Zhuo FF, Yang Z, Zhao MM, Guo Q, Liu Y, Liu D, Zeng KW, Tu PF
EBioMedicine 2022 Dec;86:104353

TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability.

Zhou S, Peng J, Xiao L, Zhou C, Fang Y, Ou Q, Qin J, Liu M, Pan Z, Hou Z
Cell death & disease 2021 May 8;12(5):463

Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.

Tong J, Xu X, Zhang Z, Ma C, Xiang R, Liu J, Xu W, Wu C, Li J, Zhan F, Wu Y, Yan H
Haematologica 2020 Jun;105(6):1630-1640

JP3, an antiangiogenic peptide, inhibits growth and metastasis of gastric cancer through TRIM25/SP1/MMP2 axis.

Chen JJ, Ren YL, Shu CJ, Zhang Y, Chen MJ, Xu J, Li J, Li AP, Chen DY, He JD, Shu YQ, Zhou JW
Journal of experimental & clinical cancer research : CR 2020 Jun 23;39(1):118

Knockdown of ZBTB7A inhibits cell proliferation of breast cancer through regulating the ubiquitination of estrogen receptor alpha.

Xiao X, Shen Y, Yin L, He J, Ni X, Luo G, Chen X, Zhu W, Zhong J, Liu J, Peng X, Zu X
Life sciences 2019 Dec 15;239:117042

ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.

Osei Kuffour E, König R, Häussinger D, Schulz WA, Münk C
mBio 2019 Aug 27;10(4)

Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15.

Teyra J, Singer AU, Schmitges FW, Jaynes P, Kit Leng Lui S, Polyak MJ, Fodil N, Krieger JR, Tong J, Schwerdtfeger C, Brasher BB, Ceccarelli DFJ, Moffat J, Sicheri F, Moran MF, Gros P, Eichhorn PJA, Lenter M, Boehmelt G, Sidhu SS
Structure (London, England : 1993) 2019 Apr 2;27(4):590-605.e5

Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.

Liu W, Li J, Zheng W, Shang Y, Zhao Z, Wang S, Bi Y, Zhang S, Xu C, Duan Z, Zhang L, Wang YL, Jiang Z, Liu W, Sun L
eLife 2017 Jun 8;6

USP15 regulates type I interferon response and is required for pathogenesis of neuroinflammation.

Torre S, Polyak MJ, Langlais D, Fodil N, Kennedy JM, Radovanovic I, Berghout J, Leiva-Torres GA, Krawczyk CM, Ilangumaran S, Mossman K, Liang C, Knobeloch KP, Healy LM, Antel J, Arbour N, Prat A, Majewski J, Lathrop M, Vidal SM, Gros P
Nature immunology 2017 Jan;18(1):54-63

Disturbed P53-MDM2 Feedback Loop Contributes to Thoracic Aortic Dissection Formation and May be a Result of TRIM25 Overexpression.

Gong B, Wang Z, Zhang M, Hu Z, Ren Z, Tang Z, Jiang W, Cheng L, Huang J, Ren W, Wang Q
Annals of vascular surgery 2017 Apr;40:243-251

Robust Lys63-Linked Ubiquitination of RIG-I Promotes Cytokine Eruption in Early Influenza B Virus Infection.

Jiang J, Li J, Fan W, Zheng W, Yu M, Chen C, Sun L, Bi Y, Ding C, Gao GF, Liu W
Journal of virology 2016 Jul 15;90(14):6263-6275

Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.

Wang P, Arjona A, Zhang Y, Sultana H, Dai J, Yang L, LeBlanc PM, Doiron K, Saleh M, Fikrig E
Nature immunology 2010 Oct;11(10):912-9

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 12573-1-AP(TRIM25 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using TRIM25 antibody 12573-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line