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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX100617 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100617, RRID:AB_1240745
- Product name
- TRIM25 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:A431 7.5% SDS PAGEGTX100617 diluted at 1:3000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody [N2C1], Internal (GTX100617) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM25 antibody [N2C1], Internal detects TRIM25 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: TRIM25 protein stained by TRIM25 antibody [N2C1], Internal (GTX100617) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using TRIM25(GTX100617) antibody at 1:500 dilution.
