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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX116209 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116209, RRID:AB_11175569
- Product name
- TRIM25 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references TRIM25 is associated with cisplatin resistance in non-small-cell lung carcinoma A549 cell line via downregulation of 14-3-3σ.
Qin X, Qiu F, Zou Z
Biochemical and biophysical research communications 2017 Nov 4;493(1):568-572
Biochemical and biophysical research communications 2017 Nov 4;493(1):568-572
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE GTX116209 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody (GTX116209) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM25 antibody detects TRIM25 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: TRIM25 protein stained by TRIM25 antibody (GTX116209) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM25?antibody immunoprecipitates TRIM25?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of TRIM25?protein by 2 £gg of TRIM25?antibody (GTX116209) 7.5% SDS-PAGE The immunoprecipitated TRIM25?protein was detected by TRIM25?antibody (GTX116209) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TRIM25(GTX116209) antibody at 1:500 dilution.
