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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00007706-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007706-M02, RRID:AB_894076
- Product name
- TRIM25 monoclonal antibody (M02), clone 5F12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant TRIM25.
- Antigen sequence
SQINGASRALDDVRNRQQDVRMTANRKVEQLQQEY
TEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQI
LLKKKSEIQTLKEEIEQSLTKRDEFEFLEK- Isotype
- IgG
- Antibody clone number
- 5F12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TRIM25 monoclonal antibody (M02), clone 5F12 Western Blot analysis of TRIM25 expression in Hela S3 NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TRIM25 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to TRIM25 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
