Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-61814 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TRIM25 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded bladder cancer tissues using TRIM25 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded rectum cancer tissues using TRIM25 mouse mAb with DAB staining.