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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [2]
- Immunohistochemistry [3]
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- Product number
- LS-C682386 - Provider product page
- Provider
- LSBio
- Product name
- TRIM25 Antibody (clone 5B5B12) LS-C682386
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5B5B12
- Storage
- Short term: store at 4°C. Long term: store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunofluorescence analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TRIM25 mouse mAb with DAB staining.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using TRIM25 mouse mAb with DAB staining.
