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Western blot
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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN501044 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Tripartite Motif Containing 25 (TRIM25) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- TRIM25 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TRIM25.
- Description
- Peptide affinity chromatography
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- C-Term
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 2 - 8°C for up to one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Efp targets 14-3-3 sigma for proteolysis and promotes breast tumour growth.
Analysis of estrogen-responsive finger protein expression in benign and malignant human breast.
Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor alpha.
Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
Urano T, Saito T, Tsukui T, Fujita M, Hosoi T, Muramatsu M, Ouchi Y, Inoue S
Nature 2002 Jun 20;417(6891):871-5
Nature 2002 Jun 20;417(6891):871-5
Analysis of estrogen-responsive finger protein expression in benign and malignant human breast.
Thomson SD, Ali S, Pickles L, Taylor J, Pace PE, Lymboura M, Shousha S, Coombes RC
International journal of cancer. Journal international du cancer 2001 Jan 15;91(2):152-8
International journal of cancer. Journal international du cancer 2001 Jan 15;91(2):152-8
Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor alpha.
Orimo A, Inoue S, Minowa O, Tominaga N, Tomioka Y, Sato M, Kuno J, Hiroi H, Shimizu Y, Suzuki M, Noda T, Muramatsu M
Proceedings of the National Academy of Sciences of the United States of America 1999 Oct 12;96(21):12027-32
Proceedings of the National Academy of Sciences of the United States of America 1999 Oct 12;96(21):12027-32
Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
Inoue S, Orimo A, Hosoi T, Kondo S, Toyoshima H, Kondo T, Ikegami A, Ouchi Y, Orimo H, Muramatsu M
Proceedings of the National Academy of Sciences of the United States of America 1993 Dec 1;90(23):11117-21
Proceedings of the National Academy of Sciences of the United States of America 1993 Dec 1;90(23):11117-21
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of TRIM25 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with AP30946PU-N at 1 ?g/ml.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunocytochemistry of TRIM25 in HeLa cells with AP30946PU-N at 20 ?g/ml.
