Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- RQ6343 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Trim25 Antibody / Efp
- Antibody type
- Polyclonal
- Description
- This highly specific Trim25 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/Direct ELISA applications with mouse and rat samples.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Trim25 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) rat RH35, 3) mouse liver and 4) mouse stomach lysate with Trim25 antibody. Predicted molecular weight ~71 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE mouse forestomach carcinoma cells (MFC) with Trim25 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse intestine tissue with Trim25 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat intestine tissue with Trim25 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse RAW264.7 cells with Trim25 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Trim25 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of rat NRK cells with Trim25 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Trim25 antibody.
