Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB118F-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-18 R beta/IL-1 R7 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-18 R beta/IL-1 R7 in direct ELISAs and Western blots. In direct ELISAs, 15% cross-reactivity with recombinant human (rh) IL-1 RII is observed and 2% cross-reactivity with rhIL-1 R9 is observed. Also in direct ELISAs, no cross-reactivity with rhIL-1 RI, rhIL-1 RacP/IL-1 R3, rhIL-1 R4, rhIL-18 R alpha/IL-1 R5, rhIL-1 R6, rhIL-1 R8, or recombinant mouse (rm) IL-18 R beta/IL-1 R7 is observed. In Western blots, approximately 100% cross-reactivity with rmIL-18 R beta/IL-1 R7 is observed and no cross-reactivity with rhIL-18 R alpha/IL-1 R5, rhIL-1 RacP/IL-1 R3, rhIL-1 RI, rhIL-1 RII, or rhIL-1 Rrp2/IL-1 R6 is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
O95256
- Isotype
- IgG
- Antibody clone number
- 132029
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-18 R beta/IL-1 R7 in KG-1 Human Cell Line by Flow Cytometry. KG-1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL-18 R beta/IL-1 R7 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB118F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.