PA5-47087

antibody from Invitrogen Antibodies

Targeting: IL18RAP AcPL, CD218b

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47087

Provider

Invitrogen Antibodies

Product name

IL18RAP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-18 R beta (AcPL), recombinant human (rh) IL-18 R, rhIL-1 R1, rhIL-1 RII, and rhIL-1 Rrp2 is observed.

Concentration

0.2 mg/mL

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of IL18RAP in KG‚1 human acute myelogenous leukemia cell line. Samples were incubated in IL18RAP polyclonal antibody (Product # PA5-47087). In the presence of Recombinant Human TNF‚α (20 ng/mL), Recombinant Human IL‚18/IL‚1F4 stimulates IFN‚γ secretion in the KG‚1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN‚γ Quantikine ELISA Kit. Under these conditions, IFN‚γ secretion elicited by Recombinant Human IL‚18/IL‚1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‚18 Rβ/IL‚1 R7 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.3-1.2 µg/mL.