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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF118 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-18 R beta/IL-1 R7 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-18 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-18 R beta (AcPL), recombinant human (rh) IL-18 R, rhIL-1 R1, rhIL-1 RII, and rhIL-1 Rrp2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O95256- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis.
de Jager W, Vastert SJ, Beekman JM, Wulffraat NM, Kuis W, Coffer PJ, Prakken BJ
Arthritis and rheumatism 2009 Sep;60(9):2782-93
Arthritis and rheumatism 2009 Sep;60(9):2782-93
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-gamma secretion Induced by IL-18/IL-1F4 and Neutralization by Human IL-18 R beta/IL-1 R7 Antibody. In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/IL-1F4 stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-18 R beta/IL-1 R7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF118). The ND50 is typically 0.3-1.2 µg/mL.
