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- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-27652 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSFM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, K562. Predicted reactivity: Mouse (89%), Rat (89%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Novel compound mutations in the mitochondrial translation elongation factor (TSFM) gene cause severe cardiomyopathy with myocardial fibro-adipose replacement.
Perli E, Pisano A, Glasgow RIC, Carbo M, Hardy SA, Falkous G, He L, Cerbelli B, Pignataro MG, Zacara E, Re F, Della Monica PL, Morea V, Bonnen PE, Taylor RW, d'Amati G, Giordano C
Scientific reports 2019 Mar 25;9(1):5108
Scientific reports 2019 Mar 25;9(1):5108
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- TSFM Polyclonal Antibody detects TSFM protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: TSFM protein stained by TSFM Polyclonal Antibody (Product # PA5-27652) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
