Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-31940 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PABPC4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji. Predicted reactivity: Human (99%), Mouse (96%), Rat (96%), Zebrafish (90%), Xenopus laevis (91%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.6 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PABPC4 using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PABPC4 polyclonal antibody (Product # PA5-31940) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PABPC4 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PABPC4 Polyclonal Antibody (Product # PA5-31940) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PABPC4 Polyclonal Antibody detects PABPC4 protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. PABPC4 Polyclonal Antibody (Product # PA5-31940) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PABPC4 Polyclonal Antibody detects PABPC4 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PABPC4 Polyclonal Antibody (Product # PA5-31940) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.