LS-C287086

antibody from LSBio

Targeting: STAG1 SA-1, SA1, SCC3A

Western blot (Enhanced validation)

Immunocytochemistry (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C287086

Provider

LSBio

Product name

STAG1 / SA1 Antibody (aa1150-1200) LS-C287086

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Goat

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human SA1 by Western Blot. Sample: Nuclear extract from ~500000 HeLa cells. Antibody: Affinity purified goat anti-SA1 used at 0.5 ug/ml. Detection: Chemiluminescence with 15 sec. exposure.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human SA1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SA1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemical Localization of Human SA1. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA1 (BL143G; ) used at 1 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemical Localization of Human SA1. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA1 (BL143G; ) used at 1 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemical Localization of Human SA1. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA1 (BL143G; ) used at 1 ug/ml.