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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB100-299 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-299, RRID:AB_10001281
- Product name
- Goat Polyclonal SA1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Xenopus
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SA1 Antibody [NB100-299] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SA1 Antibody [NB100-299] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody NB100-299 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody NB100-299 (lot NB100-299-3) used for IP at 6 ug per reaction. SA1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-299-2). For blotting immunoprecipitated SA1, NB100-299 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
