H00009111-D01

antibody from Abnova Corporation

Targeting: NMI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009111-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009111-D01, RRID:AB_10562830

Product name

NMI MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human NMI protein.

Antigen sequence

MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITK
KNIQLRKEIQKLETELQEATKEFQIKEDIPETKMK
FLSVETPENDSQLSNISCSFQVSSKVPYEIQKGQA
LITFEKEEVAQNVVSMSKHHVQIKDVNLEVTAKPV
PLNSGVRFQVYVEVSKMKINVTEIPDTLREDQMRD
KLELSFSKSRNGGGEVDRVDYDRQSGSAVITFVEI
GVADKILKKKEYPLYINQTCHRVTVSPYTEIHLKK
YQIFSGTSKRTVLLTGMEGIQMDEEIVEDLINIHF
QRAKNGGGEVDVVKCSLGQPHIAYFEE


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NMI expression in transfected 293T cell line (H00009111-T02) by NMI MaxPab polyclonal antibody.Lane 1: NMI transfected lysate(35.10 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NMI MaxPab rabbit polyclonal antibody. Western Blot analysis of NMI expression in human spleen.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NMI MaxPab rabbit polyclonal antibody. Western Blot analysis of NMI expression in mouse liver.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to NMI on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of NMI transfected lysate using anti-NMI MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NMI purified MaxPab mouse polyclonal antibody (B01P) (H00009111-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol