Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00009111-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009111-M01, RRID:AB_546530
- Product name
- Mouse Monoclonal N myc interactor Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NMI - N-myc (and STAT) interactor
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi ( Cat # H00009111-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody (M01), clone 9D8 (Cat # H00009111-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody (M01), clone 9D8.Lane 1: NMI transfected lysate(35.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - NMI monoclonal antibody (M01), clone 9D8 Analysis of NMI expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: N myc interactor Antibody (9D8) [H00009111-M01] - Detection limit for recombinant GST tagged NMI is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.