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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- A300-551A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NMI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NMI by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), 293T (T; 50 µg) and NIH3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-NMI antibody (Cat. No. A300-551A) used at 0.04 µg/ml for WB. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NMI by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and bladder carcinoma (right). Antibody: Affinity purified rabbit anti- NMI (Cat. No. A300-551A Lot1) used at a dilution of 1:1,000 (0.2 µg/ml). Detection: DAB.
