Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-763 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-763, RRID:AB_2152998
- Product name
- Rabbit Polyclonal N myc interactor Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: N myc interactor Antibody [NB100-763] - Immunoprecipitation: NMIImmunoprecipitation of Human NMI. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-NMI antibody NB100-763 used at 3 ug/ml for IP. Immunoprecipitated NMI was blotted using rabbit anti-NMI antibody (NB100-764) at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 5 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: N myc interactor Antibody [NB100-763] - Detection of human NMI/N myc interactor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NMI antibody NB100-763 (lot 2) used for IP at 6 ug per reaction. NMI was also immunoprecipitated by rabbit anti-NMI antibody NB100-763-1. For blotting immunoprecipitated NMI, NB100-763 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: N myc interactor Antibody [NB100-763] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NMI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB