LS-C757504

antibody from LSBio

Targeting: GPR45 PSP24, PSP24A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C757504

Provider

LSBio

Product name

GPR45 Antibody (aa 121-149, Internal) LS-C757504

Antibody type

Polyclonal

Description

Protein A and Peptide Affinity Chromatography

Reactivity

Human

Host

Rabbit

Storage

Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

GPR45 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GPR45 antibody detected the GPR45 protein (arrow).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

All lanes: Anti-GPR45 Antibody (Center) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-GPR45 Antibody (Center) at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.