MA5-37991

antibody from Invitrogen Antibodies

Targeting: TNPO3 IPO12, LGMD1F, MTR10A, TRN-SR, TRN-SR2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-37991

Provider

Invitrogen Antibodies

Product name

TNPO3 Recombinant Rabbit Monoclonal Antibody (0U3Q1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Samples: HeLa, RD, Jurkat, Mouse lung, Mouse testis, Mouse thymus, Rat testis Immunogen sequence: QVMNQLGQQL VSQLLHTCCF CLPPYTLPDV AEVLWEIMQV DRPTFCRWLE NSLKGLPKET TVGAVTVTHK QLTDFHKQVT SAEECKQVCW ALRDFTRLFR

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

0U3Q1

Vial size

100 µL

Concentration

1.65 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TNPO3 in extracts of various cell lines. Samples were incubated with TNPO3 Monoclonal antibody (Product # MA5-37991) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using TNPO3 Recombinant Rabbit Monoclonal Antibody (ARC2310) (Product # MA5-37991) and a 90 kDa band corresponding to TNPO3 was observed across K-562, HeLa, A549, Mouse testis and Rat testis which are high expression models and no band was seen in SK-N-AS, A-431 and Mouse heart which are low expression models. Whole cell extracts (30 µg lysate) of K-562 (Lane 1), HeLa (Lane 2), A549 (Lane 3), SK-N-AS (Lane 4), A-431 (Lane 5), Mouse Testis (Lane 6), Rat Testis (Lane 7), Mouse Heart (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1,000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of TNPO3 in U-2 OS cells using TNPO3 Recombinant Monoclonal Antibody (Product # MA5-37991) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of TNPO3 in NIH-3T3 cells using TNPO3 Recombinant Monoclonal Antibody (Product # MA5-37991) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of TNPO3 in U-2 OS cells using TNPO3 Recombinant Monoclonal Antibody (Product # MA5-37991) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of TNPO3 in NIH-3T3 cells. Samples were incubated with TNPO3 Monoclonal antibody (Product # MA5-37991) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of TNPO3 in U-2 OS cells. Samples were incubated with TNPO3 Monoclonal antibody (Product # MA5-37991) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TNPO3 in paraffin-embedded rat kidney. Samples were incubated with TNPO3 Monoclonal antibody (Product # MA5-37991) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TNPO3 in paraffin-embedded human esophageal cancer. Samples were incubated with TNPO3 Monoclonal antibody (Product # MA5-37991) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.